But Great Britain won gold to take their first ever title in the men’s 4×100 metres relay.
Running in the final leg for Jamaica, Bolt pulled him with 60m to go and tumbled to the track.
Stewards offered to help Bolt with a wheelchair but the sprint legend opted to limp away and applaud the crowd.
BBC athletics commentator Steve Cram said: “This is a sad sight. A very sad sight.
“Nobody wants to see a sportsman of that standing like this.”
Jamaica had won the last four 4x100m relays at the World Championships but Britain won thanks to a superb final leg by Nethaneel Mitchell-Blake.
Four-time Olympic gold medallist athlete Michael Johnson hailed the British team.
He said: “An amazing and outstanding performance from that British team. They beat everyone fair and square.
“They delivered on a night when everyone was here – not necessarily to see this.
“Adam Gemili was just ridiculous down the back stretch. He opened up a gap that was always going to be hard for anyone to chase down.”